Antioxidant activity, total phenolics, total flavonoids and phytochemical screening of Solanum sanctae-catharinae Dunal (Solanaceae) aerial parts
DOI:
https://doi.org/10.55892/jrg.v8i18.1974Keywords:
DPPH, TAC, Polyphenols, Alkaloids, SteroidsAbstract
Solanum sanctae-catharinae Dunal it’s a native tree from Brazilian Atlantic Forest. The Solanum genus comprises numerous species that have been found to exhibit antidiabetic, antitumor, and antimalarial properties. The current study seeks to assess the antioxidant properties, of crude extracts and crude extracts fractions of S. sanctae-catharinae aerial parts. The Folin-Ciocalteu method and the aluminum-trichloride method were used to determine TPC and TFC in crude extracts and fractions of aerial parts. Two assays were conducted to evaluate antioxidant activity: the DPPH test and the phosphomolibdenium method. Differences between sample means and control groups were determined using ANOVA (one way) with Tukey’s test coupled. Correlations were obtained using the Pearson correlation coefficient for bivariate correlations. Our findings shown that the remnant fraction of the ethanolic crude extract demonstrates the most relevant antioxidant activity, followed by the chloroform fraction of the ethanolic crude extract, the remnant fraction of the ethanolic-acetic crude extract. The presence of secondary metabolites such as phenols, flavonoids, coumarins, and alkaloids in S. sanctae-catharinae phytochemical screening suggest that this antioxidant activity can be attributed to the presence of this secondary metabolites. Furthermore, the results indicate a substantial quantity of polyphenols and flavonoids in both extracts.
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*This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001.